Molecular Formula | C14H14ClN3S |
Molar Mass | 291.8 |
Density | 1.38±0.1 g/cm3(Predicted) |
Melting Point | 190-191 °C |
Boling Point | 460.3±37.0 °C(Predicted) |
Solubility | Soluble in DMSO |
Appearance | powder |
Color | white to beige |
pKa | 14.57±0.20(Predicted) |
Storage Condition | 2-8°C |
Stability | Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 1 month. |
Sensitive | Light Sensitive |
In vitro study | CPTH2 (100 μM; 12, 24, 48 hours) causes a decrease in cell proliferation after as early as 12 h with a further significant reduction after 48 h stimulation. CPTH2 (100 μM; 12 or 48 hours) causes a comparable drop of the activity in both cell lines. CPTH2 (100 μM; 48 hours) produces a drastic increase in apoptotic/dead cell population after 48 h. CPTH2 (100 μM; 12, 24, 48 hours) shows a reduced acetylation of both global AcH3 histone and H3AcK18. CPTH2 (100 μM; 24, 48 hours) is capable to counteract invasion and migration of ccRCC-786-O cells in culture. CPTH2 (0.2, 0.5, 1 mM) inhibits the growth of a GCN5 deleted strain and a single catalytic mutant E173H. CPTH2 (0.6, 0.8 mM; for 24 hours) inhibits histone H3 acetylation in yeast cell cultures. CPTH2 inhibits the Gcn5p dependent functional network. Cell Proliferation Assay Cell Line: Papillary thyroid (K1) and clear cell Renal Cell Carcinoma (ccRCC-786-O) cell lines Concentration: 100 μM Incubation Time: 12, 24, 48 hours Result: Caused a decrease in cell proliferation after as early as 12 h with a further significant reduction after 48 h stimulation. Cell Viability Assay Cell Line: K1 and ccRCC-786-O cell lines Concentration: 100 μM Incubation Time: 24 hours (K1 cell) and 48 hours (ccRCC-786-O cell) Result: Caused a comparable drop of the activity in both cell lines. Apoptosis Analysis Cell Line: ccRCC-786-O cells Concentration: 100 μM Incubation Time: 48 hours Result: Produced a drastic increase in apoptotic/dead cell population after 48 h. Western Blot Analysis Cell Line: ccRCC-786-O cells Concentration: 100 μM Incubation Time: 12, 24, 48 hours Result: Showed a reduced acetylation of both global AcH3 histone and H3AcK18. |
WGK Germany | nwg |
biological activity | CPTH2 is an effective inhibitor of histone acetyltransferase (HAT) and can regulate Gcn5p network. CPTH2 induces apoptosis and reduces the invasiveness of the ccRCC cell line by inhibiting KAT3B. |
target | TargetValue HAT () Gcn5p () KAT3B () |
Target | Value |
in vitro study | CPTH2 (100 μ m; 12, 24, 48 hours) causesa decrease in cell proliferation after as early as 12 h with a further significant reduction after 48 h stimulation. CPTH2 (100 μ m; 12 or 48 hours) causesa comparable drop of the activity in both cell lines. CP2 (100 μ m; 48 hours) produces a drastic increase in apoptotic/dead cell population after 48 h. CPTH2 (100 μ m; 12, 24, 48 hours) shows a reduced acetylation of both global AcH3 histone and H3AcK18. CPTH2 (100 μ m; 24, 48 hours) is capable to counteract invasion and migration of ccRCC-786-O cells in culture. CPTH2 (0.2, 0.5, 1 mM) inhibits the growth of a GCN5 deleted and a single catalytic utante173h. cpth2 (0.6, 0.8mm; For 24 hours) inhibits histone H3 acetylation in east cell cultures. CPTH2 inhibits the Gcn5p dependent functional network. Cell Proliferation Assay Cell Line: Papillary thyroid (K1) and clear cell renalcell Carcinoma (ccRCC-786-O) cell lines Concentration: 100 μM Incubation Time: 12, 24, 48 hours result: used a decrease in cell proliferation after as early as 12 h with a further significant reduction after 48 h stimulation. cell Viability assaycell line: k1 and ccRCC-786-O cell lines Concentration: 100 μ m Incubation time: 24 hours (k1 cell) and 48 hours (ccRCC-786-O cell) result: used a comparable drop of the activity in both cell lines. Apoptosis Analysis cell line: ccRCC-786-O cells Concentration: 100 μM Incubation Time: 48 hours Result: Produced a drastic increase in apoptotic/dead cell population after 48 h. Western Blot Analysis Cell Line: ccRCC-786-O cells Concentration: 100 μM Incubation Time: 12, 24, 48 hours Result: Showed a reduced acetylation of both global AcH3 histone and H3AcK18. |
Cell Line: | Papillary thyroid (K1) and clear cell Renal Cell Carcinoma (ccRCC-786-O) cell lines K1 and ccRCC-786-O cell lines ccRCC-786-O cells ccRCC-786-O cells |
Concentration: | 100 μM 100 μM 100 μM 100 μM |
Incubation Time: | 12, 24, 48 hours 24 hours (K1 cell) and 48 hours (ccRCC-786-O cell) 48 hours 12, 24, 48 hours |
Result: | Caused a decrease in cell proliferation after as early as 12 h with a further significant reduction after 48 h stimulation. Caused a comparable drop of the activity in both cell lines. Produced a drastic increase in apoptotic/dead cell population after 48 h. Showed a reduced acetylation of both global AcH3 histone and H3AcK18. |